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Links to novel customer applications Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators. and
Sensitivity Analysis of Intracellular Signaling Pathway Kinetics Predicts Targets for Stem Cell Fate Control.
Jue Wang and Xin Xie at the National Center for Drug Screening, Shanghai Institute of Materia Medica, China, developed and validated an assay to find compounds that competitively bind to the EGF receptor therefore interfering with the receptor’s internalization. The assay measured the amount and intensity of intracellular fluorescent spots with the Thermo Scientific Cellomics Spot Detector BioApplication. The group screened 48,000 compounds and concluded the study with thirteen compounds with consistent inhibitory effects, one of which was a novel structure.  Download the complete article in PDF format
The Zandstra laboratory at the University of Toronto used the Thermo Scientific Cellomics ArrayScan VTI to biologically validate a computer model of leukemia inhibitor factor (LIF)-induced Jak/Stat3 pathway kinetics in embryonic stem cells. The ArrayScan provided the necessary multi-channel fluorescent image analysis needed to verify the computer model’s prediction of the self-renewal process employed by embryonic stem cells through the Jak/Stat3 pathway. As a result of the model’s predictions, the team identified novel pathway responses that combine with environmental cues to determine embryonic stem cell lineage.
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