Cellomics HCS Reagent Kit for activated (cleaved) intracellular cysteine protease Caspase 3.

Description

The Cellomics Caspase 3 Activation HCS Reagent Kits contain optimized reagents for the detection and quantitation of caspase 3 activation (cleaved) in the cells. These kits allow direct in-cell measurements using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibody is specific for cleaved caspase 3 from human, mouse and rat and does not recognize full-length caspase 3 or other caspases. The secondary antibody is conjugated to DyLight 549 Fluor (orange). The kit reagents are optimized for use with the ArrayScan HCS Reader and the Target Activation BioApplication Software Module, but they also can be used with other Cellomics BioApplication Modules (see the Compatible BioApplication Software Modules Section). Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis and management have been combined into a high-content screening (HCS) system to assay for test compounds affecting the activation of caspase 3. In addition to HCS instruments, cells labeled by the reagents in this kit can be viewed and analyzed by other fluorescence microscopes.

Capsases are intracellular cysteine proteases that are important in apoptotic cell death in a variety of cell lines. Caspase 3 can be activated by two different pathways: mitochondrial apoptosis and Fas ligand-mediated apoptosis. Caspase 3 exists as an inactive zymogen in cells and is activated by cleavage at Asp175 site by upstream caspases, caspase 9 in mitochondrial pathway and caspase 8 in Fas ligand-mediated apoptotic pathway.  Activated caspase 3 cleaves several substrates including PARP, leading to DNA fragmentation during apoptosis. Caspase 3 is also known as YAMA, Apopain or CPP32. The active caspase 3 along with the ArrayScan HCS Reader and the Target Activation BioApplication Software Module enable the quantitation of active caspase 3 in cells.

Related Poster Presentations

	Bhaskar S. Mandavilli, Krishna Vattem, and Richik N. Ghosh (2008) Automated, Quantitative, Multi-Parametric Monitoring of Different Programmed Cell Death Pathways Using Cell-Based High-Content Screening Assays.
	Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) A New Panel of High Content Screening Cell-Based Assays for in vitro Cytotoxicity Measurements.
	Jeffrey R. Haskins, Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) New reagent kits for high content cell-based assays for cytotoxicity.
	B. Nickel, C. Paroz, C. Falk, P Zbinden, U. Regenass (2006) High Content Screening for Apoptosis in Drug Discovery.

Ordering Information

Screening size kits and components (bulk and custom) available with special pricing. Please call.

To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products.

Choose "Order Online" in table to see pricing and order online (U.S. only).

See all Cellomics HCS Reagent Kits