Cellomics HCS Reagent Kit for activated (cleaved) intracellular cysteine protease Caspase 9.

Description

The Cellomics Caspase 9 Activation Reagent Kits contain optimized reagents for the detection and quantitation of caspase 9 activation in cells. These kits allow direct in-cell measurements using a fixed end-point assay based on immunofluorescence detection in cells grown on standard high-density microplates. The primary antibody is specific for human cleaved caspase 9 and does not cross-react with total caspase 9 or other caspases. The secondary antibody is conjugated to DyLight 549 Fluor (orange).

The kit reagents have been optimized for use with the ArrayScan HCS Reader and the Target Activation BioApplication Software Module, but they also can be used with other Cellomics BioApplication Modules (see the Compatible BioApplication Software Modules Section). Thus, automated plate-handling, focusing, cell image acquisition/processing, and data analysis and management have been combined into a high-content screening (HCS) system to assay for test compounds affecting the activation of caspase 9. In addition to HCS instruments, cells labeled by the reagents in this kit can be viewed and analyzed by other fluorescence microscopes.

Capsases are intracellular cysteine proteases that are important in apoptotic cell death in a variety of cell lines. Caspase 9 (ICE-LAP6, Mch6) is a member of mitochondrial pathway of apoptosis. Apoptotic induction by this pathway leads to release of cytochrome c from mitochondria that associates with pro-caspase 9 and Apaf1 leading to self cleavage of pro-caspase 9 at Asp315 and activation. The proteolytic processing of pro-caspase 9 is regulated by Akt phosphorylation at Ser196. Activated caspase 9 cleaves and activates caspase 3 leading to DNA fragmentation and apoptosis.1-3 Caspase 9 is identical to Apaf-3.

The Cellomics Caspase 9 Activation Reagent Kit along with the ArrayScan HCS Reader and the Target Activation BioApplication Software Module enable the quantitation of active caspase 9 in cells.

Related Poster Presentations

	Bhaskar S. Mandavilli, Krishna Vattem, and Richik N. Ghosh (2008) Automated, Quantitative, Multi-Parametric Monitoring of Different Programmed Cell Death Pathways Using Cell-Based High-Content Screening Assays.
	Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) A New Panel of High Content Screening Cell-Based Assays for in vitro Cytotoxicity Measurements.
	Jeffrey R. Haskins, Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) New reagent kits for high content cell-based assays for cytotoxicity.

Ordering Information

Screening size kits and components (bulk and custom) available with special pricing. Please call.

To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products.

Choose "Order Online" in table to see pricing and order online (U.S. only).

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