|
Cell Cycle BioApplication |
|
|
Catalog No. S50-0021-2
DescriptionCell cycle analysis is important for assessing potential cancer therapeutics. Current methods, including flow cytometry, require large numbers of non-adherent cells and present challenges for screening and automation. We have addressed these issues with the new Cell Cycle BioApplication. This flexible, multi-channel, High Content Screening (HCS) software module allows you to measure the cell cycle phase of individual cells and report population distributions, and to simultaneously correlate this with the presence or activation state of cell cycle-associated proteins in the same cell. Cell count and nuclear morphology are also reported. A key benefit provided by the Cell Cycle BioApplication is the simultaneous analysis of three cell cycle-associated targets and the correlation of expression level or state of these targets with each other as well as with cell cycle phase. This is illustrated for DNA content and retinoblastoma protein in Figure 2.
The results obtained by this assay are very specific, and show excellent well-to-well reproducibility, as shown below in Figure 3. The application is further enhanced by including analysis of nuclear shape, which can be used to identify damaged, necrotic, or apoptotic cells from the normal cycling population. Cell Cycle also features reference well calculation, which provides the user with an automated approach for comparison of treated wells with a user-defined control population. The percentage of cells that are different compared to a control population are then reported as a well-level assay output feature. In summary, the Cell Cycle BioApplication delivers a flexible, multi-parameter tool for detailed analysis of cell cycle distribution for populations of adherent cells. Features- Measures DNA content, and automatically identifies cell cycle position for individual cells
- Quantifies fluorescence intensity for up to three cell cycle-related targets labeled by immunofluorescence simultaneously in the same cell
- Calculates the normalized intensity between different cell cycle targets, enabling detection of changes in a cell cycle target’s state
- Analyzes subpopulations of cells belonging to different cell cycle phases for their response to additional target(s)
- Object segmentation improves detection and analysis of clumped cells
Benefits- Provides a rapid, automated method for cell cycle analysis in adherent cells
- Versatile application that can be applied to many different biological situations
- Flexibility allows scientists to design and optimize their own cell cycle assays
- Provides automatic classification and correlation of cell cycle position with quantity or state of other targets
Click the link below to view available product brochure
|
Home 
Products 
top