Reagent Kits for high-content imaging and analysis.

Description

Cellomics High-Content Screening (HCS) Reagents Kits are powerful tools for cell-based screening and analysis of specific molecular targets and biological parameters. Among these kits are those designed to measure markers that are diagnostic of specific cell cycle and proliferation states. Cellomics HCS Reagent Kits are designed and validated with the Cellomics ArrayScan HCS Reader and BioApplication Software, together providing a “Total Solution” for high-content screening. HCS Reagent Kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy.

HCS Reagent Kit Highlights

• Available in validated single- and multiplexed configurations
• Robust – Z' factor > 0.3 for both multiplexed and single-target kits
• Complete sets of critical reagents and optimized protocols included to ensure reproducible results
• Superior probes developed using DyLight Fluorescent Labeling Reagents
• Validated with Cellomics ArrayScan Instruments and BioApplication Software
• Compatible with any fluorescence HCS/HCA platform and standard fluorescence microscopy
• Customized components and bulk quantities available
• See index page for all categories of Cellomics HCS Reagent Kits

About Cell Cycle and Proliferation

At any given moment during development from a stem cell to the fully differentiated state, a normal cell is either dividing (mitosis) or not (interphase). The cell cycle is an ordered set of events, culminating in cell growth and division into two daughter cells. The cell cycle can be divided into four different phases, G1, S, G2 and M. If cells become quiescent, the cells are in G0 phase.

Cell proliferation analysis and the cell cycle characterization of agents that either promote or inhibit cell proliferation are critical areas of cell biology and drug-discovery research. The cell cycle can be analyzed by measuring DNA content and by detecting cell cycle-specific cellular targets. One example is BrdU staining that detects S phase of the cell cycle. Changes in the expression level of several protein markers are also associated with cell proliferation. For example, Ki67 is expressed during G1, S, G2, and M phases but is absent in the G0 phase. Other examples include phospho-Histone H3 (M phase marker), cyclins/Cdks, Phospho-Rb and PLK. Aberrations in cell cycle control can lead to cancer or cell death. High-content analysis (HCA) can be used to quantify cell proliferation activity by simultaneously measuring and correlating multiple parameters relating to the cell cycle.

Available Kits for Cell Cycle and Proliferation

We offer an extensive (and growing) line of optimized protocols and reagent kits for single- and multiplexed high-content analysis (HCA) of morphological, structural and phenotypic changes of whole cells and intracellular constituents.

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