Reagent Kits for high-content imaging and analysis.

Description

Cellomics High-Content Screening (HCS) Reagents Kits are powerful tools for cell-based screening and analysis of specific molecular targets and biological parameters. Among the available kits are those designed to measure key markers in the cascade of processes by which the transfer of information along a pathway generates a cellular response. Cellomics HCS Reagent Kits are designed and validated with the Cellomics ArrayScan HCS Reader and BioApplication Software, together providing a “Total Solution” for high-content screening. HCS Reagent Kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy.

Highlights

• Available in validated single- and multiplexed configurations
• Robust – Z' factor > 0.3 for both multiplexed and single-target kits
• Complete sets of critical reagents and optimized protocols included to ensure reproducible results
• Superior probes developed using DyLight Fluorescent Labeling Reagents
• Validated with Cellomics ArrayScan Instruments and BioApplication Software
• Compatible with any fluorescence HCS/HCA platform and standard fluorescence microscopy
• Customized components and bulk quantities available
• See index page for all categories of Cellomics HCS Reagent Kits

About Cell Signaling and Transcription Factors

Cell signaling – the biochemical processes by which cells communicate, transfer, and process information – is vital for proper cellular functioning. Cellular fate and tissue-specific gene expression are regulated by the activity of proteins known as transcription factors that interact with specific DNA sequences to direct the activation or repression of target genes.

Transcription factors can be selectively activated or deactivated by other proteins, often as the final step in signal transduction. Regulation of transcription can occur directly through modification of DNA, post-translation modification of histones, and/or transcription factors. Phosphorylation, acetylation, ubiquitination and other covalent modifications of proteins play an important role in most cellular processes because they can cause rapid changes in the activities of pre-existing proteins. This type of mechanism for regulating protein function is especially crucial in signal transduction pathways and in regulating the cell cycle.

One of the simpler modes of signal transmission from surface receptors to the nucleus is illustrated by proteins that bind to DNA sequences known as cAMP-response elements (CREs), the proteins being called CRE-binding proteins (CREB proteins). A more complex mechanism involving the activation of protein kinase cascades is used to transmit signals from growth-factor receptors to the immediate-early genes that are regulated by transcription factors such as AP-1 (which is a heterodimer of c-jun and c-fos). The major function of these cascades is to activate members of the mitogen-activated protein kinase (MAPK) family of serine/threonine protein kinases, and downstream protein kinases. A third mechanism for transmitting signals from the cell surface to the nucleus – first demonstrated for NFkB – is regulated nuclear translocation of transcription factors that are stored as inactive cytoplasmic complexes. Certain transcription factors such as Smads, act as transcriptional activators or repressors.

Available Kits for Cell Signaling and Transcription Factors

We offer an extensive (and growing) line of optimized protocols and reagent kits for single- and multiplexed high-content analysis (HCA) of cell signal parameters for use in cancer, CNS, cardiopulmonary, inflammation, or endocrinology research. Some relevant molecular targets and cellular signaling pathways include receptor tyrosine kinase and mitogenic signaling pathways, G-protein coupled receptor, PI3 Kinase signaling pathway, Jak-STAT, Wnt-b-catenin, NFkB, TGFb, cell and ER stress, inflammation, cell cycle and proliferation activities, apoptosis, DNA damage and repair responses.

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