Thermo Scientific Cellomics High-Content
Screening Products
arrowHome arrow Products arrow HCS Reagent Kits arrow Cell Viability Kit Part of Thermo Fisher Scientific  


 
Search

Navigation
Home
Products
HCS Reagent Kits
HCS Library
Training
Promotions
Careers

Browsing Help

The new home for Thermo Scientific Cellomics Products is www.thermo.com/hcs.

However, for a limited time, you can find existing product information & documentation on this site by browsing the above navigation menu or using the search box.

Be aware that information may not be current on this domain. We apologize for the inconvenience. We hope to have all resources updated and available on the new site in the next few months.

Please contact us if you need assistance!


 
Cell Viability Reagent Kit Print E-mail
Cellomics HCS Reagent Kit for 2-parameter (positive and negative) analysis of cell viability.
Description
The Cellomics Cell Viability HCS Reagent Kit provides high-content screening(HCS)-qualified fluorescence reagents and a validated, optimized protocol for cytotoxicity screening, using either a Cellomics fully automated HCS Reader, other HCS imaging system, or standard fluorescence microscope. At the core of the Cell Viability assay are DeadDye™ and VitalDye™ — proprietary fluorescent probes that enable quantification of the number of live and dead cells, as well as their relative percentages and live/dead ratio, on any standard microplate format.

When cells are dead (or dying) plasma membranes leak and become permeable to large macromolecules. In this state, DeadDye enters the cell and can be visualized by its red fluorescence emission (Figure 1B). VitalDye, a membrane-permeable fluorescent probe, operates as a live-cell indicator. Soluble cytoplasmic enzymes cleave VitalDye, trapping it inside the intact cells. Live cells stained with VitalDye are distinguished from dead cells by their green fluorescence emission (Figure 1B). Identification of the entire cell population is achieved using Hoechst Dye, a membrane permeable nucleic acid stain emitting in the blue fluorescence channel (Figure 1A). DeadDye and VitalDye may be used separately or in combination to quantify dead cells, live cells, or both cell populations.

Features
  • Assay is versitle and widely applicable to a variety of cell types
  • Utilizes fluorescent reagents and requires no cell lysis, purification, or filtration steps; and no handling of radioactive isotopes
  • Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS imaging systems.
  • DeadDye™ and VitalDye™ fluorescent probes offer sensitivite, quantitative and qualitative screening results without radioactivity

Kit Contents
  • VitalDye™ Fluorescent probe
  • DeadDye™ Fluorescent probe
  • Wash buffer (10x)
  • Hoechst dye
  • Positive control compound
  • Plate seals
Ordering Information

Screening size kits and components (bulk and custom) available with special pricing. Please call.

To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products.

Choose "Order Online" in table to see pricing and order online (U.S. only).

BuyProduct #DescriptionPkg. Size
Order OnlineK0200011Cell Viability (2-parameter)5 x 96
Order OnlineR0200021Cell Viability (2-parameter)50 x 96

See all Cellomics HCS Reagent Kits

 

 
Contact Us

Thermo Fisher Scientific
Cellular Imaging:
1.800.432.4091 (Toll free)
412.770.2500
Fax: 412.770.2450
E-mail


Sales Information -
Thermo Fisher Scientific
Cellular Imaging, Europe
:
+44 (0)118.988.0262
Fax: +44 (0)118.988.0362

General inquires:
1.800.432.4091
E-mail
Product Service and
Technical Support:
1-800-432-4091 - Ext. -2525
E-mail

Thermo Fisher Scientific Japan
Cellular Imaging
C-2F, 3-9 Moriya-cho
Kanagawa-ku, Yokohama
221-0022, Japan
Phone: +81-45-453-9220
Fax: +81-45-453-9222
www.cellomics.jp

 


ClubHCS

ClubHCS is a resource to help you with your HCS experience as well as the opportunity to interact with the worldwide community of HCS users.

If you currently use an ArrayScan® or KineticScan® HCS Reader or a cellWoRx™ High Content Cell Analysis System, click here to Register


 
top

Thermo Fisher Scientific Inc.