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Cell Viability Reagent Kit |
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Cellomics HCS Reagent Kit for 2-parameter (positive and negative) analysis of cell viability.DescriptionThe Cellomics Cell Viability HCS Reagent Kit provides high-content screening(HCS)-qualified fluorescence reagents and a validated, optimized protocol for cytotoxicity screening, using either a Cellomics fully automated HCS Reader, other HCS imaging system, or standard fluorescence microscope. At the core of the Cell Viability assay are DeadDye™ and VitalDye™ — proprietary fluorescent probes that enable quantification of the number of live and dead cells, as well as their relative percentages and live/dead ratio, on any standard microplate format. When cells are dead (or dying) plasma membranes leak and become permeable to large macromolecules. In this state, DeadDye enters the cell and can be visualized by its red fluorescence emission (Figure 1B). VitalDye, a membrane-permeable fluorescent probe, operates as a live-cell indicator. Soluble cytoplasmic enzymes cleave VitalDye, trapping it inside the intact cells. Live cells stained with VitalDye are distinguished from dead cells by their green fluorescence emission (Figure 1B). Identification of the entire cell population is achieved using Hoechst Dye, a membrane permeable nucleic acid stain emitting in the blue fluorescence channel (Figure 1A). DeadDye and VitalDye may be used separately or in combination to quantify dead cells, live cells, or both cell populations. Features- Assay is versitle and widely applicable to a variety of cell types
- Utilizes fluorescent reagents and requires no cell lysis, purification, or filtration steps; and no handling of radioactive isotopes
- Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS imaging systems.
- DeadDye™ and VitalDye™ fluorescent probes offer sensitivite, quantitative and qualitative screening results without radioactivity
Kit Contents- VitalDye™ Fluorescent probe
- DeadDye™ Fluorescent probe
- Wash buffer (10x)
- Hoechst dye
- Positive control compound
- Plate seals
Ordering InformationScreening size kits and components (bulk and custom) available with special pricing. Please call. To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products. Choose "Order Online" in table to see pricing and order online (U.S. only). | Buy | Product # | Description | Pkg. Size | | Order Online | K0200011 | Cell Viability (2-parameter) | 5 x 96 | | Order Online | R0200021 | Cell Viability (2-parameter) | 50 x 96 |
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