Cellomics HCS Reagent Kit for multiplex (4-parameter) analysis of cytotoxicity.

Description

The Multiparameter Cytotoxicity 1 HCS Reagent Kit is an in vitro assay tool that allows users to rapidly acquire information on the changes in the following cellular properties: (1) nuclear morphology/size, (2) cell membrane permeability, (3) lysosomal mass-pH and (4) cell density (number of cells per field). Following a toxic insult, cells may respond with changes in nuclear size and/or morphology depending on the cell type and the compound. Loss of cell membrane integrity is another common phenotypic feature of cytotoxicity. Some toxins can interfere with the cell’s functionality by affecting the pH of organelles such as lysosomes and endosomes, or by causing an increase in the number of lysosomes. The Multiparameter Cytotoxicity 1 Kit contains a cocktail of fluorescent dyes that allows one to measure these cellular properties of interest simultaneously. In addition, this assay provides information about changes in cell density (number of cells per field) that are brought about by toxic effects of the compounds tested, on cellular attachment to the assay plate matrix.

Any xenobiotic compound entering an animal is primarily targeted to the liver and kidney for metabolism and excretion. Hence, this assay was developed in cell types and/or cell lines that have been derived from these organs. The cells that could be used for this assay include but are not limited to rat primary hepatocytes, HepG2, BHK and MDCK cells. Prepared cells can be analyzed using standard fluorescence microscopy or using a Cellomics fully automated HCS Reader with the Multiparameter Cytotoxicity 1 BioApplication, affording automated plate handling, focusing, image acquisition, analysis, quantification, and data storage.

Features

• Simultaneously quantify four hallmark indicators of cytotoxicity: nuclear size/ morphology, cell membrane permeability, lysosomal mass-pH, and cell density (number of cells per field) - shortening the assay from days to hours
• Lysosomal marker makes it ideal for measuring phospholipidosis
• Multiplexed fluorescence-based assay provides sensitive quantitative and qualitative results without radioactivity
• No cell lysis, purification, or filtration steps—saving time and labor

Related Poster Presentations

	Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) A New Panel of High Content Screening Cell-Based Assays for in vitro Cytotoxicity Measurements.
	Jeffrey R. Haskins, Bhaskar Mandavilli, Megan Cuda, Chandrasekaran Vasudevan, Richik N. Ghosh (2007) New reagent kits for high content cell-based assays for cytotoxicity.
	Chris Larson, Abe Dalu, Herve Lebrec, Jon Ingelfield (2006) Comparison of Manual Flask-Based vs. Cellomics Microplate-based Cytotoxicity Assay Methods.
	Chandrasekaran Vasudevan, Jeffrey A. Haskins, William E. Burmeister (2006) Assessing Toxicity of Nanoparticles with In Vitro Cell Based Assays.
	Vivek C. Abraham, Brent Samson, Oleg Lapets and Jeffrey R. Haskins (2004) Automated Correlative Analysis of Multiparametric Cellular Responses Measured using High Content Screening.
	Vivek C. Abraham, Brent Samson, Oleg Lapets and Jeffrey R. Haskins (2004) In Vitro Analysis of Multiparametric Cytotoxicity at the Individual Cell Level.
	Brent Samson, Vivek C. Abraham, Oleg Lapets and Jeffrey R. Haskins (2004) Use of Kinetic High Content Cell-based Assays for Rapid Profiling of Compound Toxicity.
	Richik N. Ghosh, Vivek Abraham, Linnette Grove, Oleg Lapets and Jeffrey R. Haskins (2003) A Kinetic High Content Screening Assay for Intracellular Compartmental Analysis in Single Cells.
	Vivek C. Abraham, Brent Samson, Oleg Lapets and Jeffrey R. Haskins (2003) Large-Scale Investigation of Cytotoxicity Enabled by Automated Simultaneous Monitoring of Multiple Intracellular Processes in Individual Living Cells.

Kit Contents

• MPCT1 Fluor Cocktail (100x)
• Wash buffer (10x)
• Detailed protocol and assay data
• Plate seals

Ordering Information

Screening size kits and components (bulk and custom) available with special pricing. Please call.

To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products.

Choose "Order Online" in table to see pricing and order online (U.S. only).

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