Reagent Kits for high-content imaging and analysis.

Description

Cellomics High-Content Screening (HCS) Reagents Kits are powerful tools for cell-based screening and analysis of specific molecular targets and biological parameters. Among the available kits are those designed to measure genomic instability, DNA damage states and repair processes. Cellomics HCS Reagent Kits are designed and validated with the Cellomics ArrayScan HCS Reader and BioApplication Software, together providing a “Total Solution” for high-content screening. HCS Reagent Kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy.

HCS Reagent Kit Highlights

• Available in validated single- and multiplexed configurations
• Robust – Z' factor > 0.3 for both multiplexed and single-target kits
• Complete sets of critical reagents and optimized protocols included to ensure reproducible results
• Superior probes developed using DyLight Fluorescent Labeling Reagents
• Validated with Cellomics ArrayScan Instruments and BioApplication Software
• Compatible with any fluorescence HCS/HCA platform and standard fluorescence microscopy
• Customized components and bulk quantities available
• See index page for all categories of Cellomics HCS Reagent Kits

About Genotoxicity and DNA Damage

Genomic instability is a commonly observed toxicological effect in drug screening and results from DNA damage in the form of single and double strand breaks. DNA can be damaged by formation of DNA adducts, reactive oxygen species and DNA replication blockade. Accumulation of DNA damage leads to overall genomic instability and activation of cell cycle check point signaling. DNA damage in cells triggers phosphorylation of several targets involved in DNA repair and cell cycle arrest, including Histone H2AX, ATM kinase and Chk2, p53 and several others. DNA double strand break repair involves recruitment of Ku 70/80 proteins to the damage site leading to activation DNA protein kinases. Micronucleus (MN) formation is a hallmark of genetic toxicity; as such, micronuclei are used as indicators of genotoxicity caused by drug candidates or environmental toxins.

Available Kits for Genotoxicity and DNA Damage and Repair

We offer an extensive (and growing) line of optimized protocols and reagent kits for single- and multiplexed high-content analysis (HCA) of specific genotoxicity parameters.

Kit Group	Description
BrdU and Ki67 (Cell Proliferation)	BrdU (green) and Ki67 (orange) Kit BrdU (orange) Kit Ki67 (orange) Kit
Caspase 3	Cleaved Caspase 3 (orange) and nuclei (blue)
Caspase 9	Cleaved Caspase 9 (orange) and nuclei (blue)
Cell Proliferation Kits	BrdU (green) and Ki67 (orange) Kit BrdU (orange) Kit Ki67 (orange) Kit
Ku70/80	Ku70/80 heterodimer (orange) and nuclei (blue)
MDM2 and p53	MDM2 (green) and p53 (orange) Detection Kit MDM2 (orange) Detection Kit
Micronucleus	Nuclei and micronuclei (blue), cell content (orange) and membrane permeability changes (green)
Oxidative Stress	Oxidative stress (green-yellow) and nuclei (blue)
p53 and p21	p53 (orange) and p21 (green) p53 (orange) p21 (orange)
Phospho-p53 and p53	Phospho-p53 (Ser15) (orange) and p53 (green) Phospho-p53 (Ser15) (orange)
Phospho-ATM	Phospho-ATM (Ser1981) (orange) and nuclei (blue)
Phospho-ATM and p53	Phospho-ATM (green), p53 (orange) and nuclei (blue)
Phospho-Chk2	Phospho-Chk2 (Thr68) (orange) and nuclei (blue)
Phospho-H2AX	Phospho-H2AX (Ser139) (orange) and nuclei (blue)
MnSOD and Phospho-H2AX	MnSOD (orange) Phospho-H2AX (green) and nuclei (blue)

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