Complete, optimized reagent kits for high-content screening (HCS) and analysis (HCA).

Stain with high specificity...			...for high-content analysis

Description

Don't miss the complete list of kits at the bottom of this page! Cellomics High-Content Screening (HCS) Reagent Kits are powerful tools for cell-based screening and analysis of specific molecular targets and biological parameters. Cellomics HCS Reagent Kits are designed and validated with the Cellomics ArrayScan HCS Reader and BioApplication Software, together providing a “Total Solution” for high-content screening. HCS Reagent Kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy.

Highlights

• Available in validated single- and multiplexed configurations
• Robust – Z' factor > 0.3 for both multiplexed and single-target kits
• Complete sets of critical reagents and optimized protocols included to ensure reproducible results
• Superior probes developed using DyLight Fluorescent Labeling Reagents
• Validated with Cellomics ArrayScan Instruments and BioApplication Software
• Compatible with any fluorescence HCS/HCA platform and standard fluorescence microscopy
• Customized components and bulk quantities available

More About HCS Reagent Kits

Using appropriate combinations of fluorescent probes, antibodies and reagents is essential for realizing the full potential of powerful high-content screening (HCS) and high-content analysis (HCA). Cellomics HCS Reagent Kits provide just such a combination of components and optimized procedures. The easy-to-use kits simplify and streamline preparation of imaging-quality samples for automated cell-based assays. HCS Reagent Kits are an integral part of the Thermo Scientific Cellomics Total Solution HCS Platform, allowing a wide range of biologies to be investigated with ease.

Each kit is designed for a specific biology and is rigorously validated and optimized for HCS/HCA. To improve on previously available reagents, our scientists continue to develop many new probes that make use of the specific antibodies and the superior brightness and photostability of DyLight fluorophores. Multiplexed reagents are carefully tested to account for respective spectral characteristics of the fluorophores, antibody cross-reactivity, and filter selection, to achieve the highest emission of light (brightness) while minimizing background autofluorescence and non-specific cross-talk. The result is highest possible sensitivity, specificity and signal-to noise ratios, which translate into the high level of information-recovery by Cellomics ArrayScan Instruments and BioApplication Software Modules.

Even if you do not have access to high-end HCS instrumentation and analysis software, you can be confident that the high standards met by the HCS Reagent Kits for HCS also mean that they will provide for the best possible imaging with standard fluorescence microscopes. Whether your research is in cancer, toxicology, CNS, cardiopulmonology, or endocrinology, there's an HCS Reagent Kit that can carry your research forward.

Powerful Analysis Capabilities

Consider the following examples of HCS Reagent Kits in action:

• Heat Shock Proteins and Cell Stress
  

  Poster Presentation: "Detection of Cellular Stress Responses to Drugs: Heat Shock Proteins are Powerful Cell Stress Indicators in Cell-Based, High-Content Assays " by Suk J. Hong and Richik N. Ghosh

  
   
• Cell Cycle, Cell Morphology and Cytoskeletal Structure
  

  Poster Presentation: "Quantitative Analysis of Drug Effects on Cytoskeletal Structure, Cell Morphology and the Cell Cycle by High-Content Assays" by Suk J. Hong and Richik N. Ghosh


 
• Oxidative Stress and DNA Damage
  

  Poster Presentation: "Assessment of Oxidative Stress and DNA Damage in Individual Cells Using Automated, Quantitative Cell-Based Imaging" by Kay Opperman, Bhaskar S. Mandavilli, and Richik N. Ghosh


 
• Inflammatory Responses
  

  Poster Presentation: "Combining Multiplexed, High-Content Analysis with Antibody Arrays to Monitor and Correlate Intracellular and Extracellular Inflammatory Responses" by Kay Opperman, Eric Hommema, Brian Webb, and Richik N. Ghosh


 
• Cell Signaling Pathways
  

  Poster Presentation: "High-Content Analysis of Multiple Key Proteins in the AKT Signaling Pathway" by Kay Opperman, Krishna Vattem, and Richik N. Ghosh


 
• Cytotoxicity and Apoptosis
  

  Poster Presentation: "Automated, Quantitative, Multi-Parametric Monitoring of Different Programmed Cell Death Pathways Using Cell-Based High-Content Screening Assays" by Bhaskar S. Mandavilli, Krishna Vattem, and Richik N. Ghosh

Links to Available Kits

To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the US
or contact your local distributor of Thermo Scientific Products.