Reagent Kits for high-content imaging and analysis.

Description

Cellomics High-Content Screening (HCS) Reagent Kits are powerful tools for cell-based analysis of specific molecular targets and biological parameters. Among these kits are those designed to measure key markers associated with inflammation and cell stress. Cellomics HCS Reagent Kits are designed and validated with the Cellomics ArrayScan HCS Reader and BioApplication Software, together providing a “Total Solution” for high-content screening. HCS Reagent Kits are also fully compatible with other fluorescence-based HCS platforms and conventional fluorescence microscopy.

HCS Reagent Kit Highlights

• Available in validated single- and multiplexed configurations
• Robust – Z' factor > 0.3 for both multiplexed and single-target kits
• Complete sets of critical reagents and optimized protocols included to ensure reproducible results
• Superior probes developed using DyLight Fluorescent Labeling Reagents
• Validated with Cellomics ArrayScan Instruments and BioApplication Software
• Compatible with any fluorescence HCS/HCA platform and standard fluorescence microscopy
• Customized components and bulk quantities available
• See index page for all categories of Cellomics HCS Reagent Kits

About Inflammation and Cell Stress

The cells that predominate in the early phase of classical inflammation include platelets, endothelial cells and neutrophils, which are followed in later phases by macrophages, lymphocytes and fibroblasts. Inflammation plays a key role in the pathophysiology of many diseases including atherosclerosis, diabetes and Alzheimer’s disease. A number of inflammatory markers that are measurable in blood have been investigated for their ability to predict an inflammation response. These markers include IL-1β, IL-6, IL-8, IL-10, TNF-α, TGFb, EGF, PDGF and VEGF. The most popular treatment to control the inflammatory responses is use of non-steroidal inflammatory drugs that inhibit cyclooxygenase.  

In response to environmental or cellular stresses, cells initiate a program of gene expression either to cope with the stress by inducing repair mechanisms or to mark the cell for apoptosis leading to cell death. This cellular stress response is a universal mechanism of extraordinary physiological/pathophysiological significance. Compromising the integrity of lipid, protein, DNA, redox status, cell cycle control, protein folding, or any one of many other events can elicit a new program of gene expression that can induce a repair process. Common stress-inducible genes include p53, JNK, AP-1, NF-kappaB, MAPK. The stressed endoplasmic reticulum (ER) responds to misfolded proteins via the unfolded protein response pathway involving ER resident transmembrane kinases and chaperones.

Available Kits for Inflammation and Cell Stress

We offer an extensive (and growing) line of optimized protocols and reagent kits for single- and multiplexed high-content analysis (HCA) of important inflammation and cell stress targets.

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