Cellomics HCS Reagent Kit for activated nuclear factor kappa B (NFκB) and its translocation to the nucleus.DescriptionThe NFkB HCS Reagent Kit provides high-content screening (HCS) - quality fluorescence reagents and a validated protocol for measurement of Nuclear Factor kB Activation. This immunofluorescence-based reagent kit enables the measurement of the cytoplasm to nucleus translocation of NFkB (Figures 1 & 2), a critical step in the coupling of extra cellular stimuli to the transcriptional activation of specific target genes. Cytoplasm to nucleus translocation is an earlier and more definitive measure of transcription factor activation relative to electrophoretic mobility shift or gene reporter assays.
This kit can be used for identification of compounds that inhibit or induce NFkB activation on a cell-by-cell basis. Assays are performed in standard, high-density microplates, where measurements of the rate and extent of NFkB translocation are made in intact cells, providing more biologically representative information. The NFkB Activation kit combines fluorescent reagents and protocols for optimized sample preparation and assays, and requires no cell lysis, purification, or filtration steps. Prepared cells can be analyzed using standard fluorescence microscopy or using a Cellomics fully automated HCS Reader with the Cytoplasm to Nucleus Translocation Bioapplication, affording automated plate handling, focusing, image acquisition, analysis, quantification, and data storage. Features- All reagents and protocols included for optimized sample preparation
- No cell lysis, purification, or filtration steps
- Fluorescent conjugates; no handling of radioactive isotopes
- Cells prepared with the HCS Reagent Kits may be analyzed directly via standard fluorescence microscopy; or HCS imaging system
Related Poster Presentations | Kay Opperman, Eric Hommema, Brian Webb, and Richik N. Ghosh (2008) Combining Multiplexed, High-Content Analysis with Antibody Arrays to Monitor and Correlate Intracellular and Extracellular Inflammatory Responses. | | Ulla Henriksen, Kay Opperman, Jacob Fog, Richik N. Ghosh, Frosty Loechel, Morten Praestegaard (2007) Profiling of Multiple Signal Pathway Activities by Multiplexing Antibody and GFP-based Translocation Assays. | | Ulla Henriksen, Kay Opperman, Jacob Fog, Richik N. Ghosh, Frosty Loechel, Morten Praestegaard (2007) Multiplexing of Antibody and GFP-based Translocation Assays: A Pathway to Better Profiling of Cellular Signalling. | | Jason Quarles, Erica Webber, Megan Cuda, Jeff Haskins, Greg Endress (2004)
Automated Dual Antibody High-Content Screening on the BioCube System. | | Chandrasekaran Vasudevan, Patrick Maunder, Jeffrey Haskins, Natalie Fursov Mei Cong and Zhong Zhong (2004)
The Use of Division-Arrested Cells Improves the Robustness of High Content Screening Assays. | | Yih-Tai Chen, Kenneth A. Guiliano, and Jeffrey R. Haskins (2003)
High-Content Screening of Gene Knock-downs Induced with siRNA Transfection. | | Chandrasekaran Vasudevan, Oleg Lapets and Jeffrey R. Haskins (2003) Simultaneous and Quantitative Analysis of Signal Transduction Pathways with a Cell-Based High Content Screening Application. | | Yih-Tai Chen, Kenneth A. Giuliano, and Jeffrey R. Haskins (2003) Validation of siRNA Gene Knockdown Through Automated Phenotypic Analysis of Cellular Function. |
Kit Contents- NFkB primary antibody
- Alexa Fluor® 488 - conjugated secondary antibody
- Hoechst dye
- Wash buffer (10x)
- Permeabilization buffer (10x)
- Detergent buffer (10x)
Ordering InformationScreening size kits and components (bulk and custom) available with special pricing. Please call. To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products. Choose "Order Online" in table to see pricing and order online (U.S. only). See all Cellomics HCS Reagent Kits
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