Cellomics HCS Reagent Kits for fluorescent detection of any rabbit or mouse primary antibody, together with stains to characterize nuclear and whole cell morphology. | Whole Cell | Primary Antibody | | | | Stain nuclei with Hoechst Dye
(ex/em = 350/461 nm)
to characterize
nuclear morphology. | Stain entire cells with
Whole Cell Stain Green
(ex/em = 493/518 nm)
to assess cell morphology. | Detect mouse or rabbit
primary antibody with
Fluorescent Secondary Antibody
(ex/em = 550/568 nm)
to detect target of interest. |
Figure 1. Example three-parameter specific detection of nuclei, whole cells, and a rabbit primary antibody that is specific for phospho-AKT. Experiment represents AKT activation in 3T3 L1 pre-adipocytes treated with IGF-1. The three images are the same cells detected with all three stains but visualized through their separate fluorescent channels. Cells were treated for 10 minutes with IGF-1 in media containing no glucose or serum after 24 hour growth factor starvation to measure AKT activation. Hyperlinked images are two-channel color overlays of phospho-AKT plus nuclei or whole cell channels. For more complete information about this specific experiment involving AKT, see the Phospho-AKT Activation Kit. |
DescriptionThe Cellomics Toolbox Kits consist of essential cell staining reagents validated and optimized for high-content screening (HCS) assays. All of the Toolbox Kits contain Whole Cell Stain (WCS) Green and Hoechst Dye (for nuclear staining), as well as various other reagents and buffers necessary for immunofluorescence labeling and detection. The Rabbit and Mouse Antibody Detection WCS Toolbox Kits also contain a secondary antibody for detecting target-specific antibodies and are formatted for use with any mouse or rabbit antibody specific for the target of interest. Cells stained using these kits also can be imaged using fluorescence or confocal microscopy. Thus, these kits provide the critical reagents and protocolnecessary to simultaneously detect two or three staining parameters associated with most HCS applications (Figure 1). Kit Contents| Component | 8404901 | 8404902 | 8405001 | 8405002 | 8405101 | 8405102 | DyLight 549 Conjugated
Goat Anti-Rabbit IgG | -- | -- | 30 µl | 72 µl | -- | -- | DyLight 549 Conjugated
Goat Anti-Mouse IgG | -- | -- | -- | -- | 30 µl | 72 µl | | Whole Cell Stain Green | 15 µl | 50 µl | 15 µl | 50 µl | 15 µl | 50 µl | | Hoechst Dye | 30 µl | 30 µl | 30 µl | 30 µl | 30 µl | 30 µl | | Wash Buffer (10X Dulbecco's PBS) | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | | Wash Buffer II (10X Dulbecco's PBS with Tween®-20) | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | Permeabilization Buffer (10X D-PBS with 1% Triton® X-100) | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | 100 ml | | Blocking Buffer (10X) | 85 ml | 85 ml | 85 ml | 85 ml | 85 ml | 85 ml | | Thin Plate Seal Assembly | 7/pack | 7/pack | 7/pack | 7/pack | 7/pack | 7/pack |
Ordering InformationScreening size kits and components (bulk and custom) available with special pricing. Please call. To order Cellomics HCS Reagent Kits, please call 800-874-3723 in the U.S.
or contact your local distributor of Thermo Scientific Products. Choose "Order Online" in table to see pricing and order online (U.S. only). | Buy | Product # | Description | Pkg. Size | | Order Online | 8404901 | Whole Cell Stain Toolbox Kit | 1 x 96 | | Order Online | 8404902 | Whole Cell Stain Toolbox Kit | 5 x 96 | | Order Online | 8405001 | Rabbit Antibody Detection WCS Toolbox Kit | 1 x 96 | | Order Online | 8405002 | Rabbit Antibody Detection WCS Toolbox Kit | 5 x 96 | | Order Online | 8405101 | Mouse Antibody Detection WCS Toolbox Kit | 1 x 96 | | Order Online | 8405102 | Mouse Antibody Detection WCS Toolbox Kit | 5 x 96 |
See all Cellomics HCS Reagent Kits
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Last Updated ( Thursday, 30 August 2007 )
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